科研项目：

1. 深圳合成生物创新研究院交叉合作项目，2022年1月-2024年12月。（主持）

2. 深圳市微生物药物智能制造重点实验室项目，2022年1月-2023年12月。（主持）

3. 深圳湾实验室开放基金项目，“基于人工智能的酶分子设计研究”，2021年11月至2024年10月。（主持）

4. 科技部，国家重点研发计划合成生物学专项，“新分子生化反应设计与生物合成系统创建”，2020年1月至2024年12月。（课题2参与）

5. 科技部，国家重点研发计划合成生物学专项，“生物合成元器件的功能解析与智能塑造”，2019年7月至2024年6月。（课题2负责人）

发表论文：(^* 相同贡献，^#通讯作者)

1. Liu J., Lu J., Zhang C., Zhou Q., Jamieson C.S., Shang C., Houk K.N.^#, Zhou J.^#, Hu Y.^# (2023) Glycosylated enzyme- cata-lysed tandem intermolecular [4+2] cycloaddition in natural product biosynthesis. Nature Chemistry, accepted.

2. Ohashi M., Tan D., Lu J., Jamieson C.S., Kanayama D., Zhou J.^#, Houk K.N.^# and Tang Y.^# (2023) Enzymatic cis-Decalin Formation in Natural Product Biosynthesis. Journal of American Chemical Society, 145(6): 3301-3305.

3. Wang L., Zang X., Zhou, J.^# (2022) Synthetic biology: A powerful booster for future agriculture. Advanced Agarochem, 1(1): 7-11.

4. Zhang J., Hou X., Chen Z., Ko Y., Ruszczycky M.W., Chen Y., Zhou J.^#, Liu H.W.^# (2022) Dioxane Bridge Formation during the Biosynthesis of Spectinomycin Involves a Twitch Radical SAM Dehydrogenase that May Have Evolved from an Epimerase. Journal of American Chemical Society, 144(22): 9910-9919.

5. Lee Y. H., Hou X., Chen R., Feng J., Liu X., Ruszczycky M.W., Gao J. M. ^#, Wang B. ^#, Zhou J. ^#, Liu H. W. ^# (2022) Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S. Journal of American Chemical Society 144(10): 4478–4486.

6. Chen T.Y., Zheng Z., Zhang X., Chen J., Cha L., Tang Y., Guo Y. ^#, Zhou J. ^#, Wang B. ^#, Liu H.W. ^#, Chang W.C. ^# (2022) Deciphering Reaction Pathway of Mononuclear Iron Enzymes Catalyzed N-C Triple Bond Formation in Isocyanide Lipopeptides and Polyketides Biosynthesis. ACS Catalysis 12: 2270–2279.

7. Wu L., Wang Z., Cen Y. ^#, Wang B.^#, Zhou J.^# (2022) Structural insight into the catalytic mechanism of the endoperoxide synthase FtmOx1. Angewandte Chemie International Edition, 61: e202112063.

8. Wen W.-H., Zhang Y., Zhang Y-Y, Yu Q., Jiang C-C., Tang M.-C., Pu J-Y., Wu L., Zhao Y-L., Shi T.^#, Zhou J.^#, Tang G.-L.^# (2021) Reductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydroisoquinoline antibiotics. Nature Communications, 12: 7085.

9. Xie L., Zang X., Cheng W., Zhang Z., Zhou J.^#, Chen M.^#, Tang Y. ^# (2021) Harzianic Acid from Trichoderma afroharzianum Is a Natural Product Inhibitor of Acetohydroxyacid Synthase. Journal of American Chemical Society, 143: 9575-9584.

10. Liu N., Wu L., Feng J., Sheng X.^#, Li J., Chen X., Li J., Liu W., Zhou J.^#, Wu Q.^#, Zhu D.^# (2021) Crystal Structures and Catalytic Mechanism of l-erythro-3,5-Diaminohexanoate Dehydrogenase and Rational Engineering for Asymmetric Synthesis of β-Amino Acids. Angewandte Chemie International Edition, 60(18): 10203-10210.

11. Gao D., Jamieson C. S., Wang Q., Yan Y., Zhou J.^#, Houk K. N,^#, Tang Y.^# (2021) A Polyketide Cyclase That Forms Medium-Ring Lactones. Journal of American Chemical Society, 143(1): 80–84.

12. Chen T-Y., Chen J., Tang Y., Zhou J.^#, Guo Y.^#, and Chang W-C^# (2021) Current understanding toward isonitrile group biosynthesis and mechanism. Chinese Journal of Chemistry 39(2): 463-472.

13. Yan Y., Zang X., Jamieson C.S., Lin H.C., Houk K.N., Zhou J.^#, Tang Y.^#, (2020) Biosynthesis of the fungal glyceraldehyde-3-phosphate dehydrogenase inhibitor heptelidic acid and mechanism of self-resistance. Chemical Science, 11: 9554-9562.

14. Ohashi M, Jamieson C.S., Cai Y., Tan D., Kanayama D., Tang M.C., Anthony S.M, Chari J.V., Barber J.S., Picazo E., T.B. Kakule, Cao S., Garg N.K., Zhou J.^#, Houk K.N.^#, Tang Y.^#, (2020) An enzymatic Alder-ene reaction. Nature, 586: 64-69.

15. Cheng R., Wu L., Lai R., Peng C., Naowarojna N., Hu W., Li X.H., Whelan S.A., Lee N., Lopez J., Zhao C., Yong Y., Xue J., Jiang X., Grinstaff M.W., Deng Z., Chen J.S., Cui Q.^#, Zhou J.^#, and Liu P.^#, (2020) Single-step Replacement of an Unreactive C-H Bond by a C-S Bond Using Polysulfide as the Direct Sulfur Source in the Anaerobic Ergothioneine Biosynthesis. ACS Catalysis, 10: 8981-8994.

16. Dong Q., Yuan S., Wu L., Su L., Zhao Q., Wu J., Huang W.^#, Zhou J.^#, (2020) Structure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate. Bioresources and Bioprocessing 7: 37.

17. Yang X., Wu L., Li A., Ye L.^#, Zhou J.^#, Yu H.^#, (2020) Engineering of Decameric D-fructose-6-phosphate Aldolase A by Combinatorial Modulation of Inter- and Intra-subunit Interactions. Chemical Communications, 56: 7561-7564.

18. Li A., Acevedo-Rocha C.G., D’Amore L., Chen J., Peng Y., Garcia-Borràs M., Gao, C., Zhu J., Rickerby H., Osuna S.^#, Zhou J.^#, Reetz M.T.^#, (2020) Regio- and Stereoselective Steroid Hydroxylation at the C7-Position by Cytochrome P450 Monooxygenase Mutants. Angewandte Chemie International Edition, 59(30):  12499-12505. (VIP)

19. Chen T.Y., Chen J., Tang Y., Zhou J.^#, Guo Y.^#, Chang W.C.^#, (2020) Pathway from N-alkylglycine to alkylisonitrile catalyzed by iron (II) and 2-oxoglutarate dependent oxygenases. Angewandte Chemie International Edition, 59(19): 7367-7371. (VIP)

20. Cai Y., Hai Y., Ohashi M., Jamieson C.S., Garcia-Borras M., Houk K.N.^#, Zhou J.^#, Tang Y.^# (2019) Structural Basis for Stereoselective Dehydration and Hydrogen-Bonding Catalysis by the SAM-Dependent Pericyclase LepI. Nature Chemistry, 11(9): 821-820.

21. Cen Y., Singh W., Arkin M., Moody T.S., Huang M.^#, Zhou J.^#, Wu Q.^#, Reetz M.T., (2019) Directed Evolution of a Highly Active “Cysteine Lipase” with a Cys-His-Asp Catalytic Triad. Nature Communications, 10(1): 3198.

22. Xu J., Cen Y., Singh W., Fan J., Wu L., Lin X., Zhou J.^# Huang M.^#, Reetz M.R.#, Wu Q.^# (2019) Stereodivergent Protein Engineering of an Enzyme to Access All Possible Stereoisomers Bearing Multiple Stereocenters. Journal of American Chemical Society 141(19): 7934-7945.